Tucson, Arizona — The investigation into the disappearance of Nancy Guthrie has entered a critical new phase as authorities work to identify an unknown DNA profile recovered from her home and analyze surveillance footage of a masked suspect seen near the property shortly before she vanished.

Guthrie, 84, was reported missing in late January after deputies responding to her Tucson-area residence discovered blood evidence and signs of a violent struggle. The case was quickly classified as a suspected abduction, prompting coordination between the Pima County Sheriff’s Department and the Federal Bureau of Investigation.

Law enforcement officials confirmed that forensic teams recovered DNA samples from items inside the home, including gloves and other physical evidence. Preliminary analysis determined the genetic material does not match Guthrie or individuals known to have routine access to the residence. Investigators say the profile is being processed through national databases while additional testing — including advanced genealogy analysis — remains under consideration.

Surveillance footage has emerged as a central investigative lead. Video from residential doorbell cameras shows a masked individual armed with what appears to be a handgun approaching the home and tampering with the camera system shortly before Guthrie’s disappearance. Authorities have released still images publicly, describing the suspect as a male of average build carrying a black Ozark Trail backpack.

The investigation has also drawn controversy over forensic handling. Law-enforcement sources indicated that certain evidence was not immediately transferred to the FBI’s Quantico laboratory, fueling speculation about interagency friction. Sheriff Chris Nanos has rejected claims of obstruction, stating that all relevant materials are being analyzed through coordinated channels involving federal partners and contracted forensic labs.

The FBI has increased the reward for information leading to Guthrie’s recovery or an arrest, underscoring the urgency of the case as thousands of public tips continue to be reviewed.

Officials caution that while investigative tensions and public speculation persist, no verified evidence has substantiated claims of a federal cover-up. Authorities say the case remains active, complex, and evolving as forensic, digital, and witness evidence continues to be processed.