Tucson, Arizona — The investigation into the disappearance of Nancy Guthrie has intensified following confirmation that unidentified DNA evidence was recovered from her residence, adding both urgency and complexity to an already high-profile case.

Guthrie, 84, vanished from her Tucson-area home in late January under circumstances authorities describe as suspicious and likely violent. Investigators responding to the scene discovered blood evidence and signs of a struggle, prompting the Pima County Sheriff’s Department to classify the case as a suspected abduction and request federal assistance.

In a recent briefing, Sheriff Chris Nanos confirmed that forensic teams collected multiple DNA samples from the property, including material found on physical items and interior surfaces. Preliminary analysis determined that the genetic material does not match Guthrie or individuals known to have regular access to the home. Officials say the samples are now undergoing advanced testing through national and private forensic laboratories.

The disclosure has fueled public speculation — particularly online — about the existence of an unidentified suspect. However, investigators caution that unmatched DNA is not uncommon in major crime scenes and does not, on its own, establish a perpetrator’s identity.

Complicating the narrative are reports of tension between local and federal authorities regarding evidence processing. Law-enforcement sources indicated that the FBI sought to conduct testing at its Quantico laboratory, while certain materials were instead routed through an external forensic contractor. The sheriff’s office has denied obstructing federal involvement, emphasizing that cooperation remains active and that all agencies share investigative findings.

Authorities have also released surveillance images showing a masked male carrying a black Ozark Trail backpack near Guthrie’s home. Thousands of public tips have followed, though none have yet produced a confirmed suspect.

The FBI has increased the reward for information leading to Guthrie’s recovery or an arrest, underscoring the case’s national priority status.

Officials stress that the investigation remains fluid. As DNA analysis, digital forensics, and tip evaluations continue, law enforcement maintains that no definitive conclusions — or conspiracy claims — are supported by verified evidence at this stage.